ThuliumV1, Main, Exploration, bibRecord, 000C46

Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

Identifieur interne : 000C46 ( Main/Exploration ); précédent : 000C45; suivant : 000C47

Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

Auteurs : Yajie Zhang [États-Unis] ; Marie Poirer-Quinot [États-Unis] ; Charles S. Springer [États-Unis] ; James A. Balschi [États-Unis]

Source :

Journal of magnetic resonance (San Diego, Calif. : 1997) [ 1090-7807 ] ; 2010.

RBID : PMC:2885488

Abstract

This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Na_i⁺) and extracellular (Na_e⁺) ²³Na⁺ signals using their longitudinal relaxation time constant (T₁) values. Na⁺-loaded yeast cell (Saccharomyces cerevisiae) suspensions were investigated. Two types of compartmental ²³Na⁺ T₁ differences were examined: a selective Na_e⁺ T₁ decrease induced by an extracellular relaxation reagent (RR_e), GdDOTP⁵⁻; and, an intrinsic T₁ difference. Parallel studies using the established method of ²³Na MRS with an extracellular shift reagent (SR_e), TmDOTP⁵⁻, were used to validate the MRR measurements. With 12.8 mM RR_e, the ²³Na_e⁺ T₁ was 2.4 ms and the ²³Na_i⁺ T₁ was 9.5 ms (9.4T, 24°C). The Na⁺ amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RR_e or by MRS/SR_e. Without RR_e, the Na⁺-loaded yeast cell suspension ²³Na MR signal exhibited two T₁ values, 9.1 (± 0.3) ms and 32.7 (± 2.3) ms, assigned to ²³Na_i⁺ and ²³Na_e⁺, respectively. The Na_i⁺ content measured was lower, 0.88 (± 0.06); while Na_e⁺ was higher, 1.43 (± 0.12) compared with MRS/SR_e measures on the same samples. However, the measured efflux rate constant was identical. T₁ MRR potentially may be used for Na_i⁺ determination in vivo and Na⁺ flux measurements; with RR_e for animal studies and without RR_e for humans.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2885488

DOI: 10.1016/j.jmr.2010.03.018
PubMed: 20430659
PubMed Central: 2885488

Affiliations:

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<front><div type="abstract" xml:lang="en"><p id="P2">This study tested the ability of MR Relaxography (MRR) to discriminate intra- (Na<sub>i</sub>
<sup>+</sup>
) and extracellular (Na<sub>e</sub>
<sup>+</sup>
) <sup>23</sup>
Na<sup>+</sup>
 signals using their longitudinal relaxation time constant (T<sub>1</sub>
) values. Na<sup>+</sup>
-loaded yeast cell (<italic>Saccharomyces cerevisiae</italic>
) suspensions were investigated. Two types of compartmental <sup>23</sup>
Na<sup>+</sup>
 T<sub>1</sub>
 differences were examined: a selective Na<sub>e</sub>
<sup>+</sup>
 T<sub>1</sub>
 decrease induced by an extracellular relaxation reagent (RR<sub>e</sub>
), GdDOTP<sup>5−</sup>
; and, an intrinsic T<sub>1</sub>
 difference. Parallel studies using the established method of <sup>23</sup>
Na MRS with an extracellular shift reagent (SR<sub>e</sub>
), TmDOTP<sup>5−</sup>
, were used to validate the MRR measurements. With 12.8 mM RR<sub>e</sub>
, the <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
 T<sub>1</sub>
 was 2.4 ms and the <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
 T<sub>1</sub>
 was 9.5 ms (9.4T, 24°C). The Na<sup>+</sup>
 amounts and spontaneous efflux rate constants were found to be identical within experimental error whether measured by MRR/RR<sub>e</sub>
 or by MRS/SR<sub>e</sub>
. Without RR<sub>e</sub>
, the Na<sup>+</sup>
-loaded yeast cell suspension <sup>23</sup>
Na MR signal exhibited two T<sub>1</sub>
 values, 9.1 (± 0.3) ms and 32.7 (± 2.3) ms, assigned to <sup>23</sup>
Na<sub>i</sub>
<sup>+</sup>
 and <sup>23</sup>
Na<sub>e</sub>
<sup>+</sup>
, respectively. The Na<sub>i</sub>
<sup>+</sup>
 content measured was lower, 0.88 (± 0.06); while Na<sub>e</sub>
<sup>+</sup>
 was higher, 1.43 (± 0.12) compared with MRS/SR<sub>e</sub>
 measures on the same samples. However, the measured efflux rate constant was identical. T<sub>1</sub>
 MRR potentially may be used for Na<sub>i</sub>
<sup>+</sup>
 determination <italic>in vivo</italic>
 and Na<sup>+</sup>
 flux measurements; with RR<sub>e</sub>
 for animal studies and without RR<sub>e</sub>
 for humans.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Massachusetts</li>
<li>Oregon</li>
</region>
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<name sortKey="Poirer Quinot, Marie" sort="Poirer Quinot, Marie" uniqKey="Poirer Quinot M" first="Marie" last="Poirer-Quinot">Marie Poirer-Quinot</name>
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Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

Discrimination of Intra- and Extracellular 23Na+ Signals in Yeast Cell Suspensions Using Longitudinal Magnetic Resonance Relaxography

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Abstract

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